Mass spectrometry

Presentation

The Mass Spectrometry instrumental cluster is open to all partners (academic or industrial, national or international) within the framework of services or collaborations.

Expertise:

  • Measurement of molecular mass and structure identification of compounds in molecular and macromolecular chemistry
  • Study of complex mixtures in combination with separation techniques
  • Trace organic analysis
  • Analysis by direct injection or LC-MS/MS
  • Advice for access to national and European infrastructures
  • Analysis of cultural heritage samples (paleoproteomics, ZooMS ...)
  • Mass spectrometry imaging

For any analysis request, please send an email to the following address:  pole-spectrometrie-masse_at_univ-lille.fr

Location 
C4 Building, 2nd floor, office 206 – Cité Scientifique
59655 Villeneuve d’Ascq, France

 

Staff:

  • Fabrice BRAY (Head of the cluster) 
  • Stéphanie FLAMENT
  • Christophe PENVERNE

The Mass Spectrometry Division has four mass spectrometers capable of addressing questions ranging from the simplest (compound validation, mass identification) to the most complex (characterization of complex mixtures by LC-MS/MS).

 

ESI-LTQ-XL Thermo Scientific

Mass spectrometer ESI-LTQ-XL Thermo Scientific

Acquisition date: 2013
Source: ESI
Analyzer: Orbitrap
LC: HPLC U3000 RS Thermo Scientific
Fragmentation: CID, HCD
Resolution: max 120,000
Applications: Polymers, chemical molecules, HRMS, peptides, proteins, sugars, lipids, metabolites

 

HPLC U3000 RS – Thermo Fisher Scientific

HPLC chromatography for chemical analysis

Acquisition date: 2018
Source: ESI
Separation of compounds in liquid phase using C18, C4, HILIC columns, etc.
Coupled with the ESI-LTQ-XL Thermo Scientific mass spectrometer

 

MALDI-TOF/TOF ABI 4800 ABSciex

Acquisition date: 2009
Source: MALDI
Analyzer: TOF-TOF
Laser: YAG
Fragmentation: CID
Resolution: max 20,000
Applications: Polymers, chemical molecules, peptides, proteins, sugars, lipids, metabolites

 

Mass spectrometer MALDI-TOF/TOF ABI 4800 ABsciex

ESI / MALDI Solarix XR 9.4 Tesla – Bruker Daltonics

Mass spectrometer FTICR Solarix XR 9.7 Tesla

Acquisition date: 2018
Source: Nano ESI, ESI, APCI
Analyzer: FT-ICR
LC: nanoLC U3000 RSLC
Fragmentation: CID, IRMPD, ECD, ETD, SORI-CID
Resolution: max 10,000,000
Applications: Complex mixtures, polymers, chemical molecules, peptides, proteins, sugars, lipids, metabolites

 

Characterization by MALDI FTICR of Compounds Deposited on Cotton
Fingerprinting via the identification of sebacic acid using MALDI-FTICR imaging

Cotton fibre bonded to a steel plate for MALDI FTICR analysis. MALDI FTICR spectra of the compounds on the cotton

Our platform is equipped with facilities enabling the biomolecular identification of bone and dental remains … from archaeological and palaeontological sites.

Our platform is one of the few facilities in the world offering services for ZooMS analysis, sex determination and the analysis of cultural heritage samples. It houses an AB Sciex 4800+ MALDI-TOF/TOF mass spectrometer, a Bruker 9.4 T MALDI/ESI FTICR mass spectrometer and a Thermo Scientific nanoHPLC Obritrap Q-exactive Plus. The platform is fully equipped to carry out protein extraction and purification for analysis, as well as sample preparation for stable isotope analysis.

Our platform is equipped with various software packages for processing ZooMS, sex determination and palaeoproteomics data, such as mmass, PAMPA, PEAKS 13 (BSI Informatics) and Mascot V3.0.

We offer service packages or collaboration opportunities for all these analyses.

You can find the leaflets on ZooMS analysis and sex determination.